Anti-proliferative and apoptotic activity of polyphenol-rich crude methanol extract of Gracillaria edulis against human Rhabdomyosarcoma (RD) and breast adenocarcinoma (MCF-7) cell lines
| dc.contributor.author | Gunathilaka, T. | |
| dc.contributor.author | Samarakoon, K. | |
| dc.contributor.author | Ranasinghe, P. | |
| dc.contributor.author | Peiris, L D. C. | |
| dc.date.accessioned | 2022-09-06T07:16:30Z | |
| dc.date.available | 2022-09-06T07:16:30Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | Seaweeds are an important source of bioactive metabolites in the development of drugs and nutraceuticals. This study aimed to analyze the anti-proliferative and apoptotic activity of methanol extract (ME) of G.edulis on human Rhabdomyosarcoma (RD) and breast adenocarcinoma (MCF-7) cell lines. De-polysaccharide ME of G.edulis was extracted to determine the anti-proliferative and apoptotic activity. The cytotoxic activity was evaluated by MTT and neutral red uptake assay, while apoptotic activity was characterized by cellular morphology, DNA fragmentation and Caspase 3/7 assay. The results of cytotoxicity assay showed that the decrease of the percentage of cell viability in a dose-dependent manner as signified by cell death. The apoptosis was induced by activating the Caspase 3/7 pathway and the RD cell line showed prominent activation of Caspase 3 compared to the MCF-7 cells have low activation. Further, the typical DNA ladder pattern was observed in RD cells, whereas MCF-7 cells did not show a typical ladder pattern. Thus, it can be concluded that the ME of G.edulis possesses anticancer activity via activating the Caspases pathway | en_US |
| dc.identifier.citation | Gunathilaka, T., et al. (2020). Anti-proliferative and apoptotic activity of polyphenol-rich crude methanol extract of Gracillaria edulis against human Rhabdomyosarcoma (RD) and breast adenocarcinoma (MCF-7) cell lines. Proceedings 2020, 4, x. | en_US |
| dc.identifier.uri | http://dr.lib.sjp.ac.lk/handle/123456789/11948 | |
| dc.language.iso | en | en_US |
| dc.subject | G.edulis, Anti-cancer, Rhabdomyosarcoma, MCF-7, MTT, Neutral red, Caspase pathway | en_US |
| dc.title | Anti-proliferative and apoptotic activity of polyphenol-rich crude methanol extract of Gracillaria edulis against human Rhabdomyosarcoma (RD) and breast adenocarcinoma (MCF-7) cell lines | en_US |
| dc.type | Article | en_US |