A Simplified Non Radioactive DNA Probe Technique for the Field Detection of Sibling Species A of the Anopheles culicifacies (Diptera: Culicidae) Complex
| dc.contributor.author | De Silva, B.G.D.N.K. | |
| dc.contributor.author | Hapugoda, G.P.G.M.D. | |
| dc.contributor.author | Karunanayake, E.H. | |
| dc.date.accessioned | 2013-04-24T05:24:37Z | |
| dc.date.available | 2013-04-24T05:24:37Z | |
| dc.date.issued | 2010 | |
| dc.date.published | 2010 | |
| dc.description.abstract | Three cloned highly repetitive DNA sequences Rp36, Rp2l7 and Rp234 isolated from An. culicifacies Giles, sensu lato were developed as non radioactive DNA probes by using a biotinylated labeling and colorimetric detection system. These non radioactive DNA probes distinguish sibling species A from species Band C of the An. culicifacies complex in a dot blot hybridization assay using single mosquito DNA extracts diluted 50 fold. The biotinylated Rp217 probe was further assayed in a more simple procedure which involves the hybridization of blots prepared from squashed mosquito heads. This technique avoids the separate extraction of mosquito DNA and facilitates a number of samples to be processed rapidly while also allowing several field analyses to be carried out on one mosquito specimen. | en_US |
| dc.identifier.citation | De Silva, B.G.D.N.K., Hapugoda, G.P.G.M.D., & Karunanayake, E.H. (2010). A Simplified Non Radioactive DNA Probe Technique for the Field Detection of Sibling Species A of the Anopheles culicifacies (Diptera: Culicidae) Complex. Vidyodaya Journal of Science, 15(1&2), 63-72. | en-US |
| dc.identifier.uri | http://dr.lib.sjp.ac.lk/handle/123456789/1014 | |
| dc.language.iso | en | en_US |
| dc.subject | Anopheles culicifacies | en_US |
| dc.subject | Non radioactive | en_US |
| dc.subject | DNA probes | en_US |
| dc.title | A Simplified Non Radioactive DNA Probe Technique for the Field Detection of Sibling Species A of the Anopheles culicifacies (Diptera: Culicidae) Complex | en_US |
| dc.type | Article | en_US |