Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m7GpppG

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dc.contributor.authorGunawardaha, D.
dc.contributor.authorDomashevskiy, A. V.
dc.contributor.authorRodriguez, D. J.
dc.contributor.authorGoss, D. J.
dc.date.accessioned2017-09-29T06:11:31Z
dc.date.available2017-09-29T06:11:31Z
dc.date.issued2016
dc.description.abstractFluorescent mRNA molecules offer a wide range of applications for studying capping/decapping reactions, translation, and other biophysical studies. Furthermore, fluorescent tags prove invaluable for tracking RNA molecules in cells. Here, we describe an efficient synthesis of a fluorescent cap analog, anthranioyl-GTP, its purification, and in vitro cap labeling of transcribed mRNA catalyzed by the recombinant vaccinia capping enzyme to produce anthranioyl-m7GpppG-capped RNA.en_US, si_LK
dc.identifier.citationD. Gunawardaha, A. V. Domashevskiy, D. J. Rodriguez, D. J. Goss, (2016), “Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m7GpppG” Methods in Molecular Biologyen_US, si_LK
dc.identifier.urihttp://dr.lib.sjp.ac.lk/handle/123456789/5587
dc.language.isoen_USen_US, si_LK
dc.subjectFluorescenceen_US, si_LK
dc.subjectAnthranioyl-m7Gen_US, si_LK
dc.subjectAnt-GTPen_US, si_LK
dc.subjectChromatographyen_US, si_LK
dc.subjectFluorescently labeled RNAen_US, si_LK
dc.subjectCapped RNAen_US, si_LK
dc.subjectRNA labelingen_US, si_LK
dc.titlePreparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m7GpppGen_US, si_LK
dc.typeArticleen_US, si_LK

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