Purification of NADP Dependent, 1,3,6,8 -Tetrahydroxynaphthalene Reductase from Verticillium dahliae brm - 1(ATCC 44571)
| dc.contributor.author | Weerasooriya, M.K.B. | |
| dc.contributor.author | Simpson, T.J. | |
| dc.date.accessioned | 2013-04-05T05:08:31Z | |
| dc.date.available | 2013-04-05T05:08:31Z | |
| dc.date.issued | 2000 | |
| dc.date.published | 2000 | |
| dc.description.abstract | NADPH dependent 1,3,6,8- tetrahydroxynaphthalene Reductase, one of the key enzymes of 1,8-dihydroxy melanin (DHN melanin) biosynthesis was isolated from verticillium dahliae brm-I (ATCC 44571) and purified to near homogeneity (as shown by SDS-PAGE), using ammonium sulphate fractionation, ion exchange chromatography on DEAE Cellulose, FPLC Mono Q anion exchange and Superose - 12 gel filtration chromatography, Fold purification and yield of the enzyme were found to be 1423 and 0.47% respectively, Specific activity ofthe final enzyme fraction was 79,7 (nmole/ mg.min) Approximate molecular weight of the purified enzyme was determined as-24 kDa by Superose-12 gel filtration chromatography, A satisfactory HPLC based enzyme assay was also developed for thepurification of enzyme | en_US |
| dc.identifier.citation | Weerasooriya, M.K.B., & Simpson, T.J. (2000). Purification of NADP Dependent, 1,3,6,8 -Tetrahydroxynaphthalene Reductase from Verticillium dahliae brm - 1(ATCC 44571). Vidyodaya Journal of Science, 9, 1-13. | en-US |
| dc.identifier.uri | http://dr.lib.sjp.ac.lk/handle/123456789/984 | |
| dc.language.iso | en | en_US |
| dc.subject | Melanin biosynthesis | en_US |
| dc.subject | Verticillium dahliae brm-l | en_US |
| dc.subject | Tetrahydroxynaphthalene reductase | en_US |
| dc.subject | Purification | en_US |
| dc.title | Purification of NADP Dependent, 1,3,6,8 -Tetrahydroxynaphthalene Reductase from Verticillium dahliae brm - 1(ATCC 44571) | en_US |
| dc.type | Article | en_US |