IN VITRO MICROPROPAGATION OF Gyrinops walla (GAERTH.) USING LEAF DISC EXPLANTS

Abstract

Attached

Gyrinops walla (Gaertn.) (Thymelaeaceae) is a slender tree which grows in wet zone regions in Sri Lanka. Potentiality of production of resinous natural product, agarwood, which used in perfumery and medicine manufacturing, grant a great demand for this species in worldwide. In vitro micropropagation technique may be a practicable solution to provide healthy planting materials in commercial scale, in order to overcome the problems caused to the natural population due to overexploitation. Thus, using leaf disc explant, possibilities of mass propagation through in vitro techniques was explored in in vitro study. Explants were surface sterilized using 0.2% carbendazim, 10% sodium hypochlorite (Clorox) and 70% ethanol each followed by two successive washings in sterile distilled water. Murashige and Skoog (MS) basal medium supplemented with varied concentrations of different plant growth regulators, 6-benzylaminopurine (BA) and naphthaleneacetic acid (NAA) were tested for induction of calli from leaf discs. Out of different growth regulator combinations, rapid callus growth was observed in MS medium supplemented with 1.0 mg/L BA and 3.0 mg/L NAA within six weeks. Calli obtained were yellowish, compact and callus induction was mainly observed along the midrib. Calli obtain could either be used for mass propagation, through indirect organogenesis or for establishment of plant cell culture to obtain secondary metabolites. From the results obtain, it could be concluded that callus initiation is feasible through leaf disc explant