A Study on Identification of Genetic Polymorphism of Aedes aegypti Using Randomly Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR)

Abstract

Amplification of random regions of genomic DNA using 10-base primers in the randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to identify the genetic polymorphisms of Ae. aegypti mosquito populations based on genetic variation. Genomic DNA was extracted from individual mosquitoes from 10 populations of Ae. aegypti and amplified in PCR reaction using single primers of arbitrary nucleotide sequence. RAPD fragments were produced using 8 different primers. The genetic relatedness of the populations were estimated using Nei and Li pair-wise genetic distances similarity index analysis.