A Simplified Non Radioactive DNA Probe Technique for the Field Detection of Sibling Species A of the Anopheles culicifacies (Diptera: Culicidae) Complex

dc.contributor.author	De Silva, B.G.D.N.K.
dc.contributor.author	Hapugoda, G.P.G.M.D.
dc.contributor.author	Karunanayake, E.H.
dc.date.accessioned	2013-04-24T05:24:37Z
dc.date.available	2013-04-24T05:24:37Z
dc.date.issued	2010
dc.identifier.citation	De Silva, B.G.D.N.K., Hapugoda, G.P.G.M.D., & Karunanayake, E.H. (2010). A Simplified Non Radioactive DNA Probe Technique for the Field Detection of Sibling Species A of the Anopheles culicifacies (Diptera: Culicidae) Complex. Vidyodaya Journal of Science, 15(1&2), 63-72.	en-US
dc.identifier.uri	http://dr.lib.sjp.ac.lk/handle/123456789/1014
dc.description.abstract	Three cloned highly repetitive DNA sequences Rp36, Rp2l7 and Rp234 isolated from An. culicifacies Giles, sensu lato were developed as non radioactive DNA probes by using a biotinylated labeling and colorimetric detection system. These non radioactive DNA probes distinguish sibling species A from species Band C of the An. culicifacies complex in a dot blot hybridization assay using single mosquito DNA extracts diluted 50 fold. The biotinylated Rp217 probe was further assayed in a more simple procedure which involves the hybridization of blots prepared from squashed mosquito heads. This technique avoids the separate extraction of mosquito DNA and facilitates a number of samples to be processed rapidly while also allowing several field analyses to be carried out on one mosquito specimen.	en_US
dc.language.iso	en	en_US
dc.subject	Anopheles culicifacies	en_US
dc.subject	Non radioactive	en_US
dc.subject	DNA probes	en_US
dc.title	A Simplified Non Radioactive DNA Probe Technique for the Field Detection of Sibling Species A of the Anopheles culicifacies (Diptera: Culicidae) Complex	en_US
dc.type	Article	en_US
dc.date.published	2010