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Diagnosing human cutaneous leishmaniasis using fluorescence in situ hybridization

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dc.contributor.author Kaluarachchi, T. J.
dc.contributor.author Wickremasinghe, R.
dc.contributor.author Weerasekera, M.
dc.contributor.author Yasawardene, S.
dc.contributor.author McBain, A. J.
dc.date.accessioned 2022-08-24T06:44:13Z
dc.date.available 2022-08-24T06:44:13Z
dc.date.issued 2021
dc.identifier.citation Kaluarachchi, T. J., et al. (2021). Diagnosing human cutaneous leishmaniasis using fluorescence in situ hybridization. PATHOGENS AND GLOBAL HEALTH, 22021. en_US
dc.identifier.uri http://dr.lib.sjp.ac.lk/handle/123456789/11742
dc.description.abstract Cutaneous leishmaniasis (CL) is endemic in Sri Lanka. Giemsa-stained slit-skin-smears (SSSGiemsa) and histology are routinely used in diagnosis with a sensitivity of 40–70%. PCR currently has limited accessibility. Therefore, we assessed the sensitivity and specificity of a previously described fluorescence in situ hybridization assay, on skin smears and biopsy samples to overcome the limitations encountered with routine diagnostic methods. Samples from a total of 123 suspected CL patients were collected and subjected to SSSGiemsa, fluorescence in situ hybridization (FISH) on slit skin smears (SSS-FISH), formalin-fixedparaffin-embedded-tissues stained with Hematoxylin & Eosin staining (FFPE-H&E) and FISH on formalin-fixed-paraffin-embedded-tissues (FFPE-FISH). Negative controls of 61 patient samples were collected from a CL non-endemic area and subjected to the same procedures. The gold standard PCR was used as a comparator. For FISH, two previously described cyanine 3 tagged Leihsmania genus-specific probes were used. Compared to PCR, SSS-Giemsa, SSS-FISH, FFPE-H&E, and FFPE-FISH had sensitivities of 76.5%, 79.1%, 50.4% and 80.9%, respectively. Routine diagnostic tests (SSS-Giemsa and FFPE-H&E) had a specificity of 100%. SSS-FISH and FFPE-FISH had specificities of 96.7% and 93.4%, respectively. FFPE-FISH had a statistically significant higher diagnostic performance than FFPE-H&E (p < 0.001). The relative performance of SSS-Giemsa, SSS-FISH and FFPE-FISH was similar (p > 0.05 for all comparisons). We conclude that FFPE-FISH is a more accurate diagnostic tool than FFPE–H&E. SSS-FISH did not have an additional advantage over SSS-Giemsa in diagnosis. However, SSS-FISH could be recommended as a minimally invasive method in studies assessing wound healing where immunological probes are used. en_US
dc.language.iso en en_US
dc.publisher PATHOGENS AND GLOBAL HEALTH en_US
dc.subject Cutaneous leishmaniasis; fluorescence in situ hybridization; Sri Lanka en_US
dc.title Diagnosing human cutaneous leishmaniasis using fluorescence in situ hybridization en_US
dc.type Article en_US


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