Efficient preparation and properties of mRNAs containing a fluorescent cap analog: Anthraniloyl-m7GpppG

dc.contributor.author	Gunawardana, D.
dc.contributor.author	Domashevskiy, A.V.
dc.contributor.author	Gayler, K.R.
dc.contributor.author	Goss, D.J.
dc.date.accessioned	2017-10-24T04:08:43Z
dc.date.available	2017-10-24T04:08:43Z
dc.date.issued	2015
dc.identifier.citation	Gunawardana, D., Domashevskiy, A.V., Gayler, K.R., Goss, D.J. (2015). "Efficient preparation and properties of mRNAs containing a fluorescent cap analog: Anthraniloyl-m7GpppG", Vol.3 (1), pp. 01-07	en_US, si_LK
dc.identifier.uri	http://dr.lib.sjp.ac.lk/handle/123456789/6058
dc.description.abstract	Attached	en_US, si_LK
dc.description.abstract	A method has been developed for synthesising fiuorescently labeled capped mRNA. The method incorporates a I single fluorescent molecule as parit of the 5'-mRNA or oligonucleotide cap site. The fluorescent m olecule, Ant-m-GTP Is specifically Incorporated Into the cap site to yield Ant-m7GpppG-capped mRNA or oligonucleotide. Efficient capping f ! was observed with 60^100% of the RNA transcripts capped with the fluorescent m olecule. The Ant-m7G derivative, w hichhas been previously:shown to interact with the eukaryotic cap binding protein elF4E, is shown in this p ap e rto b e : ■ a substrate for the Vaccinia capping enzym e and the DCP2 decapping enzym e from Arabfdopsfs. Further, the Ant- > m7GTP-capped RNA is readily translated. This Ant-m7GTP-capped RNA provides an important tool for monitoring capping reactions, translation, and biophysical studies.
dc.language.iso	en_US	en_US, si_LK
dc.subject	capped mRNA	en_US, si_LK
dc.subject	decapping assay	en_US, si_LK
dc.subject	Fluorescence	en_US, si_LK
dc.subject	imaging	en_US, si_LK
dc.subject	RNA capping	en_US, si_LK
dc.title	Efficient preparation and properties of mRNAs containing a fluorescent cap analog: Anthraniloyl-m7GpppG	en_US, si_LK
dc.type	Article	en_US, si_LK