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Adaptation of Leishmania donovani to Cutaneous and Visceral Environments; in Vivo Selection and Proteomic Analysis

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dc.contributor.author McCall, L.I.
dc.contributor.author Zhang, W.
dc.contributor.author Dejgaard, K.
dc.contributor.author Atayde, V.D.
dc.contributor.author Mazur, A.
dc.contributor.author Ranasinghe, S.
dc.contributor.author Liu, J.
dc.contributor.author Olivier, M.
dc.contributor.author Nilsson, T.
dc.contributor.author Matlashewski, G.
dc.date.accessioned 2017-11-07T08:51:06Z
dc.date.available 2017-11-07T08:51:06Z
dc.date.issued 2015
dc.identifier.citation McCall, L.I., Zhang, W., Dejgaard, K., Atayde, V.D., Mazur, A., Ranasinghe, S., Liu, J., Olivier, M., Nilsson, T., Matlashewski, G. (2015). "Adaptation of Leishmania donovani to Cutaneous and Visceral Environments; in Vivo Selection and Proteomic Analysis", Journal of proteome research en_US, si_LK
dc.identifier.uri http://dr.lib.sjp.ac.lk/handle/123456789/6598
dc.description.abstract Attached en_US, si_LK
dc.description.abstract Leishmaniasis is a neglected tropical disease caused by Leishmania protozoa. Two main forms are found in the Old World, self-limited cutaneous leishmaniasis and potentially fatal visceral leishmaniasis, with parasite dissemination to liver, bone marrow, and spleen. The Leishmania donovani species complex is the causative agent of visceral leishmaniasis worldwide, but atypical L. donovani strains can cause cutaneous leishmaniasis. We hypothesized that L. donovani can adapt to survive in response to restrictions imposed by the host environment To assess this, we performed in vivo selection in BALB/c mice with a cutaneous L. donovani clinical isolate to select for parasites with increased capacity to survive in visceral organs. We then performed whole cell proteomic analysis and compared this visceral-selected strain to the original cutaneous clinical isolate and to a visceral leishmaniasis clinical isolate. Overall, there were no major shifts in proteomic profiles; however, translation, biosynthetic processes, antioxidant protection, and signaling were elevated in visceral strains. Conversely, transport and trafficking were elevated in the cutaneous strain. Overall, these results provide new insight into the adaptability of Leishmania parasites to the host environment and on the factors that mediate their ability to survive in different organs.
dc.language.iso en_US en_US, si_LK
dc.publisher Journal of proteome research en_US, si_LK
dc.subject visceral leishmaniasis en_US, si_LK
dc.subject proteomic analysis en_US, si_LK
dc.subject virulence en_US, si_LK
dc.subject pathogenesis en_US, si_LK
dc.title Adaptation of Leishmania donovani to Cutaneous and Visceral Environments; in Vivo Selection and Proteomic Analysis en_US, si_LK
dc.type Article en_US, si_LK


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