Purification of NADP Dependent, 1,3,6,8 -Tetrahydroxynaphthalene Reductase from Verticillium dahliae brm - 1(ATCC 44571)

dc.contributor.author	Weerasooriya, M.K.B.
dc.contributor.author	Simpson, T.J.
dc.date.accessioned	2013-04-05T05:08:31Z
dc.date.available	2013-04-05T05:08:31Z
dc.date.issued	2000
dc.identifier.citation	Weerasooriya, M.K.B., & Simpson, T.J. (2000). Purification of NADP Dependent, 1,3,6,8 -Tetrahydroxynaphthalene Reductase from Verticillium dahliae brm - 1(ATCC 44571). Vidyodaya Journal of Science, 9, 1-13.	en-US
dc.identifier.uri	http://dr.lib.sjp.ac.lk/handle/123456789/984
dc.description.abstract	NADPH dependent 1,3,6,8- tetrahydroxynaphthalene Reductase, one of the key enzymes of 1,8-dihydroxy melanin (DHN melanin) biosynthesis was isolated from verticillium dahliae brm-I (ATCC 44571) and purified to near homogeneity (as shown by SDS-PAGE), using ammonium sulphate fractionation, ion exchange chromatography on DEAE Cellulose, FPLC Mono Q anion exchange and Superose - 12 gel filtration chromatography, Fold purification and yield of the enzyme were found to be 1423 and 0.47% respectively, Specific activity ofthe final enzyme fraction was 79,7 (nmole/ mg.min) Approximate molecular weight of the purified enzyme was determined as-24 kDa by Superose-12 gel filtration chromatography, A satisfactory HPLC based enzyme assay was also developed for thepurification of enzyme	en_US
dc.language.iso	en	en_US
dc.subject	Melanin biosynthesis	en_US
dc.subject	Verticillium dahliae brm-l	en_US
dc.subject	Tetrahydroxynaphthalene reductase	en_US
dc.subject	Purification	en_US
dc.title	Purification of NADP Dependent, 1,3,6,8 -Tetrahydroxynaphthalene Reductase from Verticillium dahliae brm - 1(ATCC 44571)	en_US
dc.type	Article	en_US
dc.date.published	2000