A Study on the Use of Two Tetrazolium Salts for Determination of Serum Fructosamine

Abstract

The determination of glycated serum proteins (fructosamine) has become an important tool for assessing the glycaemic status in diabetic patients. Methods involved in the laboratory assays are based on the ability of fructosamine to act as a reducing agent in alkaline solution. A colorimetry assay method was performed to compare the reduction ability of two tetrazolium salts namely p-lodonitrotetrazolium violet (INT) and Nitroblue tetrazolium chloride (NBT) for estimation of serum fructosamine. Results revealed that the reduction ability of the ketoamine group of glycated protein (fructosamine) was more towards NBT than INT.