Abstract:
The determination of glycated serum proteins (fructosamine) has
become an important tool for assessing the glycaemic status in diabetic
patients. Methods involved in the laboratory assays are based on the ability
of fructosamine to act as a reducing agent in alkaline solution. A colorimetry
assay method was performed to compare the reduction ability of two
tetrazolium salts namely p-lodonitrotetrazolium violet (INT) and Nitroblue
tetrazolium chloride (NBT) for estimation of serum fructosamine. Results
revealed that the reduction ability of the ketoamine group of glycated protein
(fructosamine) was more towards NBT than INT.
