Abstract:
Carotenoid analyses results are subject to much variation. In this study,
problems of sampling, extraction, chromatography, spectral data, and chemical
tests are discussed together with in-vitro bioaccessibility. In addition, problems
in the in-vitro trolox equivalent antioxidant capacity assay will be discussed.
Identifying carotenoids requires uv/vis spectra, spectral databases,
chromatographic behavior on thin layer chromatography (TIc), high
performance liquid chromatography (HPLC), open column chromatography
(OCC) and chemical tests all of which must be used in conjunction.
Quantification can be done by OCC and HPLC but correction factors are
needed in the latter.
