Abstract:
Candida albicans is responsible for the majority of invasive fungal infections in preterm infants.
Biofilm formation within indwelling central venous catheter (CVC) used for these preterm babies
leads to CVC related infections and may lead to antifungal resistance. Metabolically generated CO2
can act as a communicating molecule triggering the yeast to filamentous transition that is essential
for the pathological effects caused by C. albicans. The aim of this study was to demonstrate that CO2
generated by C. albicans contributes to biofilm-formation in central venous catheters utilised in
preterm infants. An in vitro model with neonatal CVCs, using established CO2 bio indicator strain
(CO2-BIS) to determine whether there is an accumulation of metabolic CO2 within the catheters,
which may be influencing C. albicans biofilm formation was developed. Biofilms formed within CVC
lines showed an enhanced recovery of CO2-BIS (p=0.06) when co-incubated with another CO2 donor
C. albicans strain CAI4. This indicated that the metabolically generated CO2 from neighbouring CAI4
cells within the biofilm had generated sufficient CO2 to complement the growth demand of CO2-BIS.
Therefore, these results highlight the importance of high concentrations of CO2 in the circulatory
system, which enhances fungal pathogenicity and may lead to invasive candidiasis.
