Abstract:
Chili pepper (Capsicum spp.)is a vegetable crop with medical and ornamental uses. In Sri Lanka, 56% of
dried chili is imported annually making it a huge burden to the economy. To improve the chili pepper
industry, breeding could be suggested as one of the strategies. With the advancement of Molecular Biology,
breeding is now practiced with marker assisted selection. According to the present knowledge, no such
breeding programs are taking place in Sri Lanka. Therefore the objectives of the present study were to
characterize the morphology and validate fruit size and shape linked microsatellite markers using an ex situ
Capsicum collection in comparison to the commercial cultivars. The accessions and cultivars were
established in a greenhouse at Peradeniya, Sri Lanka. Leaf and fruit morphological data were recorded at
flowering and fruit maturity respectively. Five microsatellite markers (HpmsE045, CAeMS010, GPMS178,
CAMS451 and CAMS493) were used to genotype all 49 accessions using PCR and bands were size separated
using polyacrylamide gel electrophoresis. According to the fruit shape, six groups were identified namely
Triangular, Elongated long, Elongated short, Spherical sharp-end, Spherical blunt-end and Rectangular.
The plant height had a negative correlation with the number of fruits of the plant at first harvest. Number of
seeds per fruit and the fruit diameter were significantly associated with the fruit weight (P<0.05).
Microsatellite marker analysis revealed 44 alleles explaining very high level of genetic diversity (in the
range of 62% to 87%). Out of the 44 alleles, 15 alleles were found to be significantly affecting on fruit size
traits and 23 alleles were associated with fruit shape. This validated marker information could be used to
plan future breeding programs and genetic studies for chili pepper.
