Abstract:
In view of worldwide interest and greater public awareness in finding plant products as the most promising and ecologically safer alternatives for synthetic insecticides in post-harvest protection of stored rice, present study was undertaken to explore the potential of leaves of Ruta graveolens (Aruda) as a repellent against S.oryzae infestations. Leaf powder and solvent extracts of R. graveolens were evaluated for their contact and fumigant repellent properties against seven day old S. oryzae adults. In contact repellency test, weevils (20 each) were exposed to 1.0 g, 3.0 g, 5.0 g, and 7.0 g of leaf powder mixed with white raw rice grains in a modified cup bioassay apparatus. Fumigation repellency of leaf powder was tested using same dosages and the number of weevils in a fumigation-repellency chamber. Number of weevils that moved from the bioassay chamber was recorded one hour after weevil introduction. Leaves of R. graveolens were extracted in hexane, ethyl acetate, methanol, distilled water and different concentrations 10, 50, 100% (v/v) were assessed separately to evaluate repellent activity by means of an area preference bioassay. In all experiments, ten weevils or each were tested and the number repelled was recorded 30 minutes after weevil introduction. Highest contact and fumigant repellent effects were elicited by 7.0 g of leaf powder resulting 96% and 95% respectively, whilst lowest dose also produced more than 50% repellency indicating extremely strong repellent action of the plant powder. In comparison, aqueous extract exhibited the most potent repellent activity (91%) while other extracts were producing over 70% repellent effects on weevils at the concentration of 100% (v/v). Overall findings of the study suggest that both powder and extracts of R. graveolens leaves could be used as eco-friendly agents for post-harvest rice protection.
