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Judo 40 is a widely used organophosphorus
pesticide in agriculture and information on male
reproductive effects is limited. Hence, the present study
has evaluated the effects of Judo 40 human sperm
function in vitro. Human sperm were incubated with
equivalent doses of 0 (conttol; cornoil) 20 and 50 mg/kg
Judo 40 for 5 min, 15 min and 30 min and percentage
motility and vitality was evaluated. Rats were monitored
for water intake and subsequent to autopsy, organ body
weights, sperm count, sperm motility, sperm proteins, pH
of seminal vesicle fluid, sperm vitality, and sperm DNA
damage was examined. Water intake of rats were
significantly reduced at both dose levels on posttreatment day 1 but returned to normal levels from day 2.
Result showed significant declining of sperm motility at
both doses (20 mg/kg: by 37.24%; 50 mg/kg by 51.24%).
pH of seminal vesicle fluid was decreased at both dose
levels. The sperm count and organ body weights remain
unaltered following the treatment. Similarly, DNA
damages were significantly increased at low dose by
95.9% and at high dose by 51.31%. Human sperm motility
was significantly reduced at low dose by 48.8% after 15
min and by 69.6% after 30 min incubation. With the high
dose motility was significantly impaired by 78.3%, 78.8%
and 83.4% after 5 min, 15 min and 30 min incubation
respectively. However, human sperm vitality was
significantly decreased only in high dose by 48.8% after
15 min incubation and by 52.1% after 30 min incubation.
The observations suggested that exposure to Judo 40 may
result in detrimental effects on both rat and human
sperm function.
