Exposure of Judo 40 Alters Dna Integrity and Sperm Function of Rat and Human Spermatozoa

Abstract

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Judo 40 is a widely used organophosphorus pesticide in agriculture and information on male reproductive effects is limited. Hence, the present study has evaluated the effects of Judo 40 human sperm function in vitro. Human sperm were incubated with equivalent doses of 0 (conttol; cornoil) 20 and 50 mg/kg Judo 40 for 5 min, 15 min and 30 min and percentage motility and vitality was evaluated. Rats were monitored for water intake and subsequent to autopsy, organ body weights, sperm count, sperm motility, sperm proteins, pH of seminal vesicle fluid, sperm vitality, and sperm DNA damage was examined. Water intake of rats were significantly reduced at both dose levels on posttreatment day 1 but returned to normal levels from day 2. Result showed significant declining of sperm motility at both doses (20 mg/kg: by 37.24%; 50 mg/kg by 51.24%). pH of seminal vesicle fluid was decreased at both dose levels. The sperm count and organ body weights remain unaltered following the treatment. Similarly, DNA damages were significantly increased at low dose by 95.9% and at high dose by 51.31%. Human sperm motility was significantly reduced at low dose by 48.8% after 15 min and by 69.6% after 30 min incubation. With the high dose motility was significantly impaired by 78.3%, 78.8% and 83.4% after 5 min, 15 min and 30 min incubation respectively. However, human sperm vitality was significantly decreased only in high dose by 48.8% after 15 min incubation and by 52.1% after 30 min incubation. The observations suggested that exposure to Judo 40 may result in detrimental effects on both rat and human sperm function.