Attached
Objectives: Objective of this study was to establish an in vitro biofilm on the 96 well plates and
to determine the efficacy of three different culture media on biofilm formation of Candida albicans
and C. tropicalis
Methods: A 96 well sterile, polystyrene plate was inoculated using 10^6 cell/ml of C. albicans
and C. tropicalis suspensions and the growth rate of planktonic cells was determined by measuring
the absorbance (OD492) at 2 hour intervals. Adhesion of Candidial cells to initiate the biofilm
formation in the presence of three culture media (Yeast Nitrogen Base (YNB) supplemented with
100 mM glucose, Sabouraud Dextrose Broth (SDB) and RPMI1640) was quantified using MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and Crystal Violet (CV) assay
after 90 minutes. Biofilms of C. albicans, C. tropicalis and 1:1 co-biofilms were developed and
the growth rates were quantified at 24 hours’ time intervals. Scanning electron microscope (SEM)
was performed to assess the architecture.
Results: Planktonic cells of both C. albicans and C. tropicalis showed maximum growth with
SDB. C. albicans and co-biofilm adhesion were significantly facilitated with RPMI1640 and the
best medium for C. tropicalis adhesion was YNB. Biofilms showed the maximum growth rate in
RPMI 1640. C. tropicalis exhibited the minimum growth with all three culture media.
Conclusions: The maximum growth rate for planktonic C. albicans and C. tropicalis was achieved
with SDB. However RPMI 1640 was the best medium for growth of biofilms
