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Introduction
As biofilms are ubiquitous life forms, biofilm
formation may vary under different
nutritional conditions such as fermentable
sugars leading to many oral complications
including dental caries and periodontal
diseases. In the present study we
investigated the effect of two different
concentrations of sugars and an artificial
sweetener on biofilm formation of Candida
species.
Objectives
The aim of the study to investigate the effect
of two different concentrations of sugars and
an artificial sweetener on biofilm formation of
Candida species. Method
The growth rates of planktonic cells of
C.albicans and C.tropicalis was determined by
inoculating 96-well plates with 106 cells/ml of
organisms suspended in 5% and 10%
sweetening agents supplemented medium
and measuring the optical density (OD492) at 2
hour intervals.
C.albicans, C.tropicalis and 1:1 mixed species
adhesion and Biofilm growth rate assays in
the presence of 5% and 10% glucose, sucrose
and saccharin were quantified using MTT (3-
(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide) and Crystal
Violet (CV). The Biofilm architecture was
assessed using Scanning electron microscopy
(SEM).
Results
Highest growth rate of planktonic Candida
was observed with 5% glucose regardless of
species. No significant growth was observed
with 5% and 10% saccharin. While both
glucose and sucrose (5% and 10%) showed
significant positive effect on adhesion of
C.albicans, C.troplicalis and mixed species, 5%
saccharin significantly reduced the adhesion
of monospecies of C.albicans and C.tropicalis.
A maximum Biofilm growth rate was observed
with 5% sucrose for all species. While 5%
saccharin had no significant effect, 10%
saccharin reduced the growth of all three
Biofilms.
Conclusions
5% saccharin did not promote the adhesion of
C. albicans and mixed species, however 10%
saccharin promoted the adhesion. Although
10% saccharin promoted the adhesion it did
not facilitate the growth of Candida biofilms.
