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The objective of the present study is to evaluate the a-amylase inhibitory and antioxidant activities, as well as
the total phenolics content of methanol (80:20 v/v) and aqueous bark extracts of T. orientalis through in vitro
analyses. Preliminary phytochemical profiling was performed to identify the bioactive constituents. Toxicity
screening was also determined through brine shrimp lethality assay. Phytochemical screening revealed the
presence of various chemical compounds with some identified phytoconstituents using GC-MS. Data indicated
that methanol extract exhibited high DPPH scavenging ability and a-amylase inhibitory activity with IC50 values
of 48.40 pg/ml and 127.56 pg/ml, respectively. Furthermore, higher total phenolics content was determined in
the methanol extract than the aqueous extract. The results suggest that the bioactivify could be due to the
synergistic effect of the active compounds, with emphasis on the phenolic compounds (phenols, flavonoids and
tannins). The methanol fraction was also shown to have a weak cytotoxicity against brine shrimp. However, the
aqueous extract prepared through decoction revealed to be non-toxic, thus supporting the safety of traditional
preparation. The combined effects of a-amylase inhibitory and antioxidant activities of bark extract of T.
orientalis may indicate a good antidiabetic potential of the plant
