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Veterinary antibiotics are physiologically highly active substances and being used on a large
scale us therapeutic drugs and feed additives in modern agricultural practice. The major purposes
are prevention and treatment of bacteria-borne diseases and improved growth rales of farm animals.
Therefore, the present study was focused on quantification of some selected Tetracycline (namely
Tetracycline; TET, and Oxytetracyeline; OTC) and some selected Penicillin (namely Amoxicillin;
AMX, and Amphiciliin; AMP) and isolation of the antibiotic resistant bacteria. Twenty wastewater
discharge drains in large-scale livestock and poultry farms were selected to collect samples for the
study. Solid-Phase Extraction (SPE) was employed and quantification was done by High
Performance Liquid Chromatography (HPLC). Isolation of resistance (r) bacteria was followed by
standard pour plate method where Minimum Inhibitory Concentration (MIC) of bacteria was
determined using 6 0 -7 2 0 ppm concentrations of antibiotics by 96-w'ell plate method and pour
plate method. OTC (5596), and TET (3896) were recorded as most frequent antibiotic in samples
and they were detected as 0.005 ppm and 0.004 ppm respectively. The highest concentration of
AMX was recorded as 0.003 ± 0.004 ppm. However, AMP was not detected during the study
period. OTC and TET resistance bacteria were identified as Enterococcus sp., Enterococcus
I'aecium, Escherichia coli and Clostridium sp. while, Acinetobactcr baumanii, Enterolvictcr clocttc.
Acinciohttcier. IwolTii and Helicobacter.pylori were identified as AMP and AMX resistance bacteria
by 16S rRNA sequencing. The MIC values of tetracycline ( I'ET, OTC) resistance bacteria ranged
from 360 ppm to 720 ppm whereas for penicillin (AMX, AMP) was from 360 to 760 ppm.
