Attached
Most triazine derivatives show various biological
applications. In this study two Zn(II) complexes were
synthesized by using the commercially available
ligand, ferene [3-(2-pyridyl-5,6-di(2-furyl)-1,2,4-
triazine- p, p'disulfonicacid disodium saltJ.The mono
complex, Zn(ferene)Cl2(l), was synthesized by
reacting starting material (ZnCl2: Ferene) in a 1:1 ratio
and the bis complex, Zn(ferene)2Cl2(2) was synthesized
by reacting starting material (ZnCl2: Ferene) in a 1: 2
ratio, respectively (Scheme 1). Both the synthesized
complexes are highly soluble in water, making them
good candidates for bio assays. The synthesized
complexes have been characterized by spectroscopic
techniques. According to the UV- Vis spectra for all the
complexes (1 and 2) a bathochromic shift has been
observed (Figure 1). Also FTIR data provide evidence
that Zn-N bonds are formed via triazine and pyridine N
atoms. Accordingly the stretching frequency of N=N
and C=N bonds of complexes have been shifted to the
low frequency range in comparison with the free
ligand. 'H NMR spectra were obtained for both
complexes and confirmed their purity. Thereafter, the
binding studies of mononuclear zinc(II) complexes (1
and 2) and ferene ligand with bovine serum albumin
(BSA) have been investigated by UV-Vis absorption
spectroscopy. With increasing the concentrations of
ferene and complexes (1) and (2) the maximum
absorbance was increased without any shift in position
of die curve. These results indicate the interaction with
BSA changing the polarity of the microenvironment
around tryptophan and tyrosine amino acid residue.
The binding constants (Kb) for ferene, complexes 1 and 2 with BSA were calculated as 3.09* 104M ‘, 12.30 *
104M'‘ and 16.84 x 104M ‘, respectively.
