attached
The optimum extraction conditions for the highest recovery of total phenolics and carotenoids
contents for leaves of Passiflora edulis were developed using response surface methodology. The
effects of solvent concentration (30-100%), extraction temperature (30-60°C) and extraction time
(30-90 min) on the recovery of total phenolics and carotenoids were investigated. A second order
polynomial model produced a satisfactory fitting of the experimental data with regard to total
phenolics (R2
= 84.75%, p < 0.004) and carotenoid (R2
= 78.74, p < 0.019) contents. The optimum
extraction conditions of ethanol concentration, extraction temperature and extraction time for
phenolics, were 6.1%, 70.2°C, and 110.5 min and for ca rotenoids, the optimum parameters were 100%, 70.2°C and 110.5 min, respectively. The optimal predicted contents for total phenolics (9.03
mg Gallic Acid Equivalent (GAE)/ g DW) and carotenoids (8.74 mg/g DW) values in the extracts
were agreed with the experimental values obtained with optimum extraction conditions for each
response.
